HiTrap rProtein A FF column

Introduction

HiTrap rProtein A FF columns are prepacked with cytiva rProtein A Sepharose™ Fast Flow resin for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow resin by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The column can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTA™.

Features

Quick, convenient preparative purification of monoclonal antibodies.
Well-established protein A-based resin used in many approved MAb processes.
Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
Simple operations with a syringe, pump, or high-performance chromatography system such as ÄKTA™ systems.

Table 1. Characteristics of HiTrap columns.

Table 2. HiTrap rProtein A FF characteristics

Table 3. Affinity of protein A for selected classes of monoclonal antibodies.

To Order

Documents

Protocol (PDF):	HiTrap rProtein A FF
MSDS (PDF):	MSDS-HiTrap rProtein A FF

Related Products

Buy	Cat.No.	Product name	Size	Price
	HP001	HiTrap rProtein A FF	1 × 1 mL	$200
	HP002	HiTrap rProtein A FF	1 × 5 mL	$750
	HP003	HiTrap rProtein A FF	5 × 1 mL	$800
	HP004	HiTrap rProtein A FF	5 × 5 mL	$3000