HiTrap DEAE Sephadex A-25 are ready-to-use 5 mL columns prepacked with cytiva DEAE Sephadex A-25 resin for small-scale purification using weak anion exchange resin.
DEAE Sephadex A-25 is weak anion exchanger. The ion exchange group is diethylaminoethyl which remains charged and maintains consistently high capacity below pH 9.
The special design of the column, together with the well-established chromatography resin DEAE Sephadex A-25, provides fast, reproducible, and easy separations in a convenient format. The column can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTA™.
Table 1. Characteristics of HiTrap columns.
Features
Table 2. HiTrap DEAE Sephadex A-25 characteristics
