Biotin-11-UTP

Biotin-11-UTP (cat. no. C404) is supplied as a 10 mM solution in TE buffer.

Biotin-11-UTP can be enzymatically incorporated into RNA with T7, T3 and SP6 RNA polymerases , as well as other RNA modifying enzymes. The number '11' is the number of atoms in the linker between biotin and UTP. Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods.

Specifications: Concentration: 10 mM solution in TE buffer Storage: Store at -20°C Molecular Formula: C28H42N6Na3O18P3S Molecular Weight: 944.62

Applications

Indirect non-radioactive enzymatic labeling of RNA during in vitro transcription.