Cy®5 Streptavidin is prepared by covalently labeling streptavidin with Cy®5 dye. Streptavidin has high binding affinity with biotin, so streptavidin conjugate is commonly used together with biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules. Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.
Reconstitution and Storage: Store lyophilized power at -20ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg streptavidn or 250 µL for 500 µg streptavidn) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
Immunofluorescent analysis of α-tubulin with Cy®5 streptavidin
|Physical State:||Lyophilized power|
|Buffer:||PBS, pH 7.4, with 0.1% BSA|
|Preservative:||0.02% sodium azide|
|Storage Conditions:||-20ºC, store in dark|
|Unit Size:||100 µg, 500 µg|
|Western Blot (WB)||1:5,000 – 1:20,000|
|Immunofluorescence (IF)||1:100 – 1:2,000|
|Immunocytochemistry (ICC)||1:100 – 1:2,000|
|Immunohistochemistry (IHC)||1:100 – 1:2,000|
|Flow Cytometry (FACS)||1:100 – 1:1,000|