Cy3 Picolyl Azide

Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.

Picolyl azide is a functionalized organic azide derivative that incorporates a picolyl group (a pyridine ring attached to a methylene group) which can chelate copper ions, enhancing the rate and efficiency of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions.

The Cy3 picolyl azide is reactive with terminal alkyne via a copper-catalyzed click reaction at a much lower copper (I) concentration without sacrificing reaction efficiency, which protects against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).

Features

 
Figure 1. Click chemistry labeling

Specifications

Label:	Cy3
Ex/Em:	555/565 nm
Detection Method:	Fluorescent
Solubility:	DMSO, DMF
Product Size:	0.5 µmol
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications Bioconjugation, biomolecular labeling, live-cell imaging, and flow cytometry.