Andy Fluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody

Introduction

Andy Fluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody is prepared by labeling high quality goat anti-rabbit IgG (H+L) with Andy Fluor™ 555. Andy Fluor™ 555 is a superior in fluorescent brightness and photostability. Andy Fluor™ 555 can be used as a replacement for Cy® 3.

Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.

Specifications		Form Information
Host/Isotype:	Goat	Physical State:	Lyophilized power
Class:	Polyclonal	Shipping Condition:	Ambient
Type:	Secondary Antibody	Purification:	Immunoaffinity chromatography
Tested Species Reactivity:	Rabbit (Rb)	Buffer:	PBS, pH 7.4, with 0.1% BSA
Target Class:	IgG (H+L)	Preservative:	0.02% sodium azide
Antibody Form:	Whole Antibody	Storage Conditions:	4ºC; store in dark
Label:	Andy Fluor™ 555
Excitation/Emission:	553/565
Unit Size:	100 µg, 500 µg

Applications	Dilution
Western Blot (WB)	1:5,000 – 1:20,000
Immunofluorescence (IF)	1:100 – 1:2,000
Immunocytochemistry (ICC)	1:100 – 1:2,000
Immunohistochemistry (IHC)	1:100 – 1:2,000
Flow Cytometry (FACS)	1:100 – 1:1,000

Immunofluorescent analysis of α-tubulin and CCSP with Andy Fluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody

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Documents

Protocol (PDF):	L114A	L114B
MSDS (PDF):	L114A	L114B
COA (PDF):	L114A	L114B

Related Products

Buy	Cat.No.	Product name	Ex/Em (nm)	Size	Price
	L114A L114B	Andy Fluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody	553/565	100 µg 500 µg	$44 $88