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HRP Goat Anti-Rabbit IgG (H+L) Antibody

Introduction

HRP Goat Anti-Rabbit IgG (H+L) Antibody is prepared from antibodies that have been affinity purified. HRP Goat Anti-Rabbit IgG (H+L) Antibody should be suitable for western blot, ELISA, immunohistochemistry and other standard immunoassay applications. The sensitivity of each lot of antibody is confirmed using ELISA. The specificity of each lot of antibody is confirmed by isoelectric focusing (IEF).

Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.

Specifications   Form Information
Host/Isotype: Goat   Physical State: Lyophilized power
Class: Polyclonal   Shipping Condition: Ambient
Type: Secondary Antibody   Purification: Immunoaffinity chromatography
Tested Species Reactivity: Rabbit (Rb)   Buffer: PBS, pH 7.6, with 1% BSA
Target Class: IgG (H+L)   Preservative: None
Antibody Form: Whole Antibody   Storage Conditions: 4ºC; store in dark
Label: HRP    
Excitation/Emission:

Western blot detection with HRP Goat Anti-Rabbit IgG (H+L) Antibody

Unit Size: 100 µg, 500 µg
   
Applications Dilution
Western Blot (WB) 1:10,000 – 1:200,000
Immunocytochemistry (ICC) 1:500 – 1:5,000
Immunohistochemistry (IHC) 1:500 – 1:5,000
ELISA 1:5,000 – 1:100,000
   

To Order

Buy Cat.No. Product name Ex/Em (nm) Size Price

L153A
L153B
HRP Goat Anti-Rabbit IgG (H+L) Antibody 50 µg
250 µg
$30
$60

Documents

Protocol (PDF): L153A L153B
MSDS (PDF): L153A L153B
COA (PDF): L153A L153B