ER-Track Red dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER). ER-Track Red dye rarely stains mitochondria, unlike the conventional ER stain DiOC6 (3), and staining at low concentrations does not appear to be toxic to cells. When cells are stained using the optimized protocol provided, staining patterns are partially retained after treatment with formaldehyde. This stain consists of the red-fluorescent BODIPY TR dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K+ channels which are prominent on ER. ER-Track Red dye has an excitation and emission maximum of 587/615 nm and can be efficiently excited using a TRITC filter. The ER-Track probes are also available in green color: ER-Track Green.
| Specifications: |
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| Excitation/Emission: | 587/615 nm | |
| Shipping Condition: | Ambient | |
| Storage Conditions: | -20ºC, protect from light | |
| Molecular Formula: | C46H46BClF2N6O7S2 | |
| Molecular Weight: | 943.29 | |
| Protocol (PDF): | C065 |
| MSDS (PDF): | MSDS-C065 |
| COA (PDF): | C065 |
Reference
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Authors: Don AS, Martinez-Lamenca C, Webb WR, Proia RL, Roberts E, Rosen H
Journal: J Biol Chem
PubMed ID: 17400555
Zinc is a novel intracellular second messenger.
Authors: Yamasaki S, Sakata-Sogawa K, Hasegawa A, Suzuki T, Kabu K, Sato E, Kurosaki T, Yamashita S, Tokunaga M, Nishida K, Hirano T
Journal: J Cell Biol
PubMed ID: 17502426
