ER-Track Green dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER). ER-Track Green dye rarely stains mitochondria, unlike the conventional ER stain DiOC6 (3), and staining at low concentrations does not appear to be toxic to cells. When cells are stained using the optimized protocol provided, staining patterns are partially retained after treatment with formaldehyde. This stain consists of the green-fluorescent BODIPY FL dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K+ channels which are prominent on ER. ER-Track Green dye has an excitation and emission maximum of 504/511 nm and can be efficiently excited using a FITC filter. The ER-Track probes are also available in red color: ER-Track Red.
| Specifications: |
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| Excitation/Emission: | 504/511 nm | |
| Shipping Condition: | Ambient | |
| Storage Conditions: | -20ºC, protect from light | |
| Molecular Formula: | C37H42BClF2N6O6S | |
| Molecular Weight: | 783.10 | |
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| Protocol (PDF): | C064 |
| MSDS (PDF): | MSDS-C064 |
| COA (PDF): | C064 |
Reference
Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis.
Authors: Mirnikjoo B, Balasubramanian K, Schroit AJ,
Journal: J Biol Chem
PubMed ID: 19561081
Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia.
Authors: Zhang H, Bosch-Marce M, Shimoda LA, Tan YS, Baek JH, Wesley JB, Gonzalez FJ, Semenza GL,
Journal: J Biol Chem
PubMed ID: 18281291
