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Bradford Protein Assay Kit

Introduction

The Bradford Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic coomassie-dye reagent changes color from brown to blue in proportion the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA). The kit includes Bradford Protein Assay Reagent and BSA standard. It is compatible with many of the salts, solvents, buffers, reducing chemicals and chelating agents often used in protein samples. Assays can be performed in cuvettes, or microplates.
 

Features

  • Bradford reagent: stable, ready-to-use kit
  • Colorimetric: measure with a standard spectrophotometer or plate reader (595 nm)
  • Easy to use: single reagent; no working reagent preparation required
  • Fast: almost immediate color development; add, mix and read results
  • Broad range: detects protein concentration in the range 1 to 1500 µg/mL
  • Flexible: microplate or cuvette format
 

product Image

BSA standard curve

Applications

Protein quantitation in solution.

To Order

Buy Catalog # Product Name Description Size List Price
P010 Bradford Protein Assay Kit Protein quantitation 500 mL $75

Documents

Protocol (PDF): P010
MSDS (PDF):  MSDS-P010