Introduction
5-FAM-X-dUTP (cat. no. C406) is supplied as a 1 mM solution in TE buffer.
5-FAM-X-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between FAM and dUTP can improve the incorporation efficiency.
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Specifications:
Excitation/Emission (nm): 495/518 nm |
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Applications
Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.
To order
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