5-FAM-X-dUTP, 1 mM solution

5-FAM-X-dUTP (cat. no. C406) is supplied as a 1 mM solution in TE buffer.

5-FAM-X-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between FAM and dUTP can improve the incorporation efficiency.

Specifications: Excitation/Emission (nm): 495/518 nm Concentration: 1 mM solution in TE buffer Storage: Store at -20°C Molecular Formula: C39H38N4Na3O21P3 Molecular Weight: 1060.62

Applications

Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.