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Dear Scientist,
Do you test your cell cultures for mycoplasma comtamination? ABP Biosciences is proud to introduce three fast and sensitive mycoplasma detection methods: MycoCheck™ Mycoplasma PCR Detection Kit, MycoCheck™ Mycoplasma Luminescent Detection Kit, and MycoCheck™ Mycoplasma Stain Kit.
The MycoCheck™ Mycoplasma PCR Detection Kit allows for fast and reliable identification of mycoplasma contamination in cell cultures. Mycoplasma DNA in the cell culture supernatant is amplified via PCR and visualized using gel electrophoresis. In addition to the short detection process (less than 2 hours), the easy handling and high sensitivity makes this Mycoplasma PCR Detection Kit a convenient tool for routine examination of cell cultures and media.
Figure 1. The PCR fragments of the commonly encountered mycoplasmas in cell cultures
The MycoCheck™ Mycoplasma Luminescent Detection Kit allows for quick and reliable screening of cell cultures for contamination with mycoplasmas. The assay utilizes the activity of mycoplasma-specific enzymes and detects through the luminescence reaction catalyzed by ATP-dependent luciferase. The entire detection process has only two steps and only takes about 15 minutes.
Figure 2. The kinetics of ATP generation in the presence of M.hyorhinis contamination
The MycoCheck™ Mycoplasma Stain Kit provides an ultrasensitive, rapid and simple fluorescence microscopic assay for the visual identification of mycoplasma infection in laboratory cell cultures. In order to detect mycoplasma, the fluorescent MycoFluor reagent is added directly to the culture medium, and the stained sample is then examined under a fluorescence microscope. Mycoplasma staining with MycoFluor reagent appears as a fine particulate or filamentous staining over the cytoplasm at 100X magnification.
Figure 3. The Jurkat cell culture stained with MycoCheck™ Mycoplasma Stain Kit
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