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Cell Proliferation Assay

Introduction

 
Cell proliferation analyses are crucial for cell growth and differentiation studies, and are often used to evaluate both compound toxicity and inhibition of tumor cell growth during drug development. Proliferation measurements are typically made based on average DNA content or on cellular metabolism. Assays can report either total cell numbers or live cells, or measure DNA synthesis in single cells.
ABP Biosciences has recently introduced a wide selection of validated products to measure cell proliferation by techniques including tracing cell generation, tracking new DNA synthesis, DNA content, or metabolic activity. These cell proliferation assay kits can be adapted to imaging, microplate, or flow cytometry assay platforms.

Tracking Cell Proliferation by Generation after Generation

Our Cell-ID™ Cell Proliferation Kits allow scientists to detect and quantify cell proliferation through multiple generations.

Cell-ID™ CFSE Cell Proliferation Kit
Cell-ID™ Violet Cell Proliferation Kit

   

Tracking New DNA Synthesis

Our iClick™ EdU assays are designed to analyze the rate of new DNA synthesis based on incorporation of the nucleoside analog EdU into DNA. Detection is achieved through a copper-catalyzed “click” reaction that is complete typically within 30 minutes.

iClick™ EdU Andy Fluor™ 488 Imaging Kit
iClick™ EdU Andy Fluor™ 555 Imaging Kit
iClick™ EdU Andy Fluor™ 594 Imaging Kit
iClick™ EdU Andy Fluor™ 647 Imaging Kit

   

Tracking New RNA Synthesis

Our iClick™ EU assays utilize an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and powerful click chemistry to detect newly synthesized RNA in a simple, two-step procedure.

iClick™ EU Andy Fluor™ 488 Imaging Kit
iClick™ EU Andy Fluor™ 594 Imaging Kit

   

Cell Proliferation Assay based on Cellular DNA Content

Our Cell-Quant™ No Wash Cell Proliferation Assay Kit provide an accurate microplate–based fluorescence method for counting cells in a population, based on cellular DNA content.

Cell-Quant™ No Wash Cell Proliferation Assay Kit

   

Measure Cell Viability and Proliferation based on Metabolic Activity

Our Cell Counting Kit-8 and Cell-Quant™ AlamarBlue Cell Viability Reagent utilize metabolic indicators to measure cellular reduction potential.

Cell Counting Kit-8
Cell-Quant™ MTT Cell Proliferation Assay Kit

Cell-Quant™ AlamarBlue Cell Viability Reagent

 

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
A001 Cell-ID™ CFSE Cell Proliferation Kit 490/517 1000 assays $180
A002 Cell-ID™ Violet Cell Proliferation Kit 405/450 200 assays $180
A003 iClick™ EdU Andy Fluor™ 488 Imaging Kit 495/520 50 assays $320
A004 iClick™ EdU Andy Fluor™ 555 Imaging Kit 555/565 50 assays $320
A005 iClick™ EdU Andy Fluor™ 594 Imaging Kit 590/615 50 assays $320
A006 iClick™ EdU Andy Fluor™ 647 Imaging Kit 650/665 50 assays $320
A007 iClick™ EdU Andy Fluor™ 488 Flow Cytometry Assay Kit 495/520 50 assays $350
A008 iClick™ EdU Andy Fluor™ 647 Flow Cytometry Assay Kit 650/665 50 assays $350
A009 iClick™ EU Andy Fluor™ 488 Imaging Kit 495/520 50 assays $320
A010 iClick™ EU Andy Fluor™ 594 Imaging Kit 590/615 50 assays $320
A011 BrdU   50 mg $80
A012 EdU   50 mg $80
A013 EU   50 mg $80
A014 Cell-Quant™ No Wash Cell Proliferation Assay Kit 490/520 1000 assays $260
A014-1 CCK-8   100 assays $30
A014-2 CCK-8   500 assays $100
A015 Cell-Quant™ MTT Cell Proliferation Assay Kit 570/- 1000 assays $180
A016-1 Cell-Quant™ AlamarBlue Cell Viability Reagent 530-560/590 500 assays $50
A016-2 Cell-Quant™ AlamarBlue Cell Viability Reagent 530-560/590 2500 assays $160