Annexin V Binding Assay

 Dear Scientist,

During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, exposing PS to the external cellular environment where it can be detected by annexin V conjugate. ABP Biosciences is proud to offer fluorescent annexin V conjugates and kits for studying the externalization of PS in apoptotic cells. These conjugates and kits are very useful for flow cytometry or fluorescence microscopy to assess mixed populations of apoptotic and nonapoptotic cells.  

 Features:

• Sensitivity: Andy Fluor™ dye conjugates for brighter signals.
• Flexibility: Conjugates available for different lasers and detection channels .
• Simplicity: Assay procedure typically completed in less than 30 minutes. 

Figure 1. Jurkat cells treated with 10 μM camptothecin for four hours (right panel) or untreated (as control, left panel). Cells were then treated with the reagents in the kit (A025), followed by flow cytometric analysis.

Related Products

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• Mitochondrial Membrane Potential Assay