The Super ECL Ultra Western Blotting Detection Kit is a super sensitive, luminol-based enhanced chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The Super ECL Ultra Western Blotting Detection Kit enables femtogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence that can be visualized on X-ray film or other imaging system. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed. The special formulation of Super ECL Ultra Substrate makes it an ideal substitute for Amersham ECL Select Substrate without the need for additional optimization of assay conditions.
The Super ECL Ultra Western Blotting Detection Kit consists of Luminol/Enhancer solution and Peroxide solution. The working ECL substrate solution is prepared by combining equal volumes of both solutions. The ECL substrate produces a high intensity signal for detection of medium to low abundant proteins.
Features
- High sensitivity ̶ detect femtogram amounts of target protein on nitrocellulose or PVDF membrane
- High signal stability ̶ incubated blots provide stable signal duration over 4 hour time period
- Stable reagent ̶ reagent is stable for 1-year
- Economical ̶ use much less antibody
The comparsion of sensitivity of Super ECL, Super ECL Ultra, and Millipore Immobilon™ Western blot substrate.
The comparsion on signal duration.
The comparsion on antibody dilution.
| Protocol (PDF): | FP302 | FP303 |
| MSDS (PDF): | MSDS-FP302 | MSDS-FP303 |
| COA (PDF): | COA-FP302 | COA-FP303 |
Cited Reference:
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Journal of Surgical Research, 2020, 256, 584-594, https://doi.org/10.1016/j.jss.2020.06.062
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Wang Z., Wu L., Pan B., Chen Y., Zhang T., Tang N.
Toxicology, 2021, 456, 152788. https://doi.org/10.1016/j.tox.2021.152788
Effect of activation of the Akt/mTOR signaling pathway by EEF1A2 on the biological behavior of osteosarcoma
Yang J, Tang J, Li J, Cen Y, Chen J, Dai G.
Ann Transl Med. 2021, 9(2):158. doi: 10.21037/atm-20-7974.
Veratrilla baillonii Franch exerts anti-diabetic activity and improves liver injury through IRS/PI3K/AKT signaling pathways in type 2 diabetic db/db mice
Cai-Jing He, Li-Qun Ma, Muhammad Sarfaraz Iqbal, Xian-Ju Huang, Jun Li, Guang-Zhong Yang, Awais Ihsan
Journal of Functional Foods, 2020, 75, 104204, https://doi.org/10.1016/j.jff.2020.104204.
Effect of Cyclic Heat Stress on Hypothalamic Oxygen Homeostasis and Inflammatory State in the Jungle Fowl and Three Broiler-Based Research Lines
Brugaletta G, Greene E, Ramser A, Maynard CW, Tabler TW, Sirri F, Anthony NB, Orlowski S, Dridi S.
Front Vet Sci. 2022, 9:905225. doi: 10.3389/fvets.2022.905225
Autophagy mediates bronchial cell malignant transformation induced by chronic arsenic exposure via MEK/ERK1/2 pathway
Linqing Wu, Zengbin Wang, Xiaotong Li, Xiaoli He, Yanfei Han, Yizhong Chen, Liangying Liu, Lengxi Fu, Tao Zhang
Toxicology Letters, 2020, 332, 155-163, https://doi.org/10.1016/j.toxlet.2020.06.006
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