ExoFast™ Exosome Isolation Reagent from other body fluids enables fast and efficient enrichment of intact exosomes from cerebrospinal fluid (CSF), ascitic fluid, amniotic fluid, milk, and saliva with a protocol that is scalable depending on your sample size.
- Maximize recovery of intact exosomes for any type of downstream application
- Easily isolate exosomes using our simple and reliable protocol
- Avoid time-consuming ultra-centrifugation
- Maximize flexibility—can be scaled up or down depending on the sample size
By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the body fluid, and the solution is incubated at 2–8°C or room temperature (temperature and length of incubation depend on specific body fluid). The precipitated exosomes are recovered by standard centrifugation at 10,000 x g. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods.