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Cell-Quant™ No Wash Cell Proliferation Assay Kit

Introduction

Our Cell-Quant™ No Wash Cell Proliferation Assay Kit provides an accurate microplate–based fluorescence method for counting cells in a population, based on cellular DNA content. Our Cell-Quant™ Assay provides a very convenient workflow for high-throughput screening of cell proliferation and cytotoxicity–no washes, cell lysis, temperature equilibrations, or radioactivity required. The Cell-Quant™ Assay has a linear detection range from less than 100 to 20,000 cells per well in most cell types. The add-mix-read assay format makes it ideal for HTS applications. The assay can be completed in one hour, and the signal is stable for several hours, affording additional work flow convenience.

Features

  • Simple workflow: No washes, cell lysis, temperature equilibrations, or radioactivity required
  • Fast: Results in as little as 60 minutes
  • High sensitivity: Detect as low as 100 cells
  • Broad dynamic range: 100-20,000 cells

Specifications

Platform: Fluorescence plate reader
Detection Method: Fluorescent
Ex/Em: 490/520 nm
Product Size: 1000 assays

Applications

Cell proliferation and cytotoxicity analysis


Figure 1. Cell-Quant™ No Wash Cell Proliferation Assay Workflow


Figure 2. Quantitation of CHO cells using the Cell-Quant™ No Wash Cell Proliferation Assay Kit. Fluorescence measurements were made using a microplate reader with excitation at 485 nm and emission detection at 530 nm. The linear range of the assay under these conditions is from 100 to 20,000 cells per 100 µl sample. The inset shows the linearity that can be obtained at very low numbers of cells.

To Order

Buy Cat.No. Product Name Ex/Em Unit Price
A014 Cell-Quant™ No Wash Cell Proliferation Assay Kit 490/520 1000 assays $260

Documents

Protocol (PDF): A014
MSDS (PDF):  MSDS-A014
   

Reference:

Application of a high-content multiparameter cytotoxicity assay to prioritize compounds based on toxicity potential in humans.

Abraham VC, Towne DL, Waring JF, Warrior U, Burns DJ,
J Biomol Screen (2008) 13:527-537

Activator of G protein signaling 3 promotes epithelial cell proliferation in PKD.

Nadella R, Blumer JB, Jia G, Kwon M, Akbulut T, Qian F, Sedlic F, Wakatsuki T, Sweeney WE, Wilson PD, Lanier SM, Park F,
J Am Soc Nephrol (2010) 21:1275-1280