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GAPDH Rabbit Polyclonal Antibody

Introduction

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

Storage Condition and Buffer: 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Application: WB IHC ICC/IF FC.

Reactivity: Human Mouse Rat Chicken Cow Dog Monkey

Dilution: WB 1:3000~1:10000 IHC 1:100~1:250 ICC/IF 1:100~1:250.

Purification: Affinity-chromatography.

Specificity: GAPDH Antibody detects endogenous levels of total GAPDH.

Immunogen: A synthesized peptide derived from human GAPDH.


Western blot analysis of GAPDH expression in (1) HeLa cell lysate; (2) MDBK cell lysate; (3) COS-1 cell lysate; (4) MDCK cell lysate with GAPDH Antibody.

Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody.

Immunofluorescent analysis of Hela cells, using GAPDH Antibody.
 

To Order

Buy Cat.No. Product name Description Size Price
AT003 GAPDH Rabbit Polyclonal Antibody Control antibody 100 µL $180

 
 
 

Documents

Protocol (PDF): AT003