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GAPDH Mouse Monoclonal Antibody

Introduction

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

Storage Condition and Buffer: 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Application: WB IHC ICC/IF IP FC.

Reactivity: H M R Mk Ch Ze Fish

Dilution: WB 1:3000~1:10000 IHC 1:100~1:500 ICC/IF 1:100~1:250 IP 1:50 FC 1:50.

Purification: Affinity-chromatography.

Specificity: GAPDH Mouse Monoclonal Antibody detects endogenous levels of total GAPDH Mouse Monoclonal.

Immunogen: A synthesized peptide derived from human GAPDH Mouse Monoclonal.


Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Mouse Monoclonal Antibody.

Immunohistochemical analysis of paraffin-embedded human colon cancer, using GAPDH Mouse Monoclonal Antibody.

Immunofluorescent analysis of Hela cells, using GAPDH Mouse Monoclonal Antibody.
 

To Order

Buy Cat.No. Product name Description Size Price
AT004 GAPDH Mouse Monoclonal Antibody Control antibody 100 µL $180

 
 
 

Documents

Protocol (PDF): AT004