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EasyProbes™ Cell Viability Imaging Kit (Blue/Green)

Introduction

EasyProbes™ Cell Viability Imaging Kit (Blue/Green) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of EasyProbes™ Hoechst 33342 Live Cell Stain reagent and EasyProbes™ Green 488 Dead Cell Stain reagent to 1 mL of cell growth media, then determine viability by counting total vs dead cells. EasyProbes™ Hoechst 33342 reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. EasyProbes™ Green 488 Dead reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard FITC (green) filter set. This kit is appropriate for fluorescence microscopy and flow cytometry.

 

  • EasyProbes™ Hoechst 33342 reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 350/461 nm)
  • EasyProbes™ Green 488 Dead reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard FITC (green) filter set (excitation/emission maxima: 500/530 nm)


Suggestions for use

  • EasyProbes™ Hoechst 33342 Live Cell Stain reagent and EasyProbes™ Green 488 Dead Cell Stain reagent may be added directly to cells in full growth media or a compatible buffer solution.
  • In most cases, 2 drops/mL and an incubation time of 10 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
Specifications:  
Detection Method: Fluorescent
Shipping Condition: Ambient
Storage Conditions: 2~8ºC, protect from light
Compatible Cells: Eukaryotic Cells, Mammalian Cells
Product Size: 2 × 10 mL dropper bottles  

 

To Order

Buy Cat.No. Product Name Ex/Em(nm) Size Price
A020 EasyProbes™ Cell Viability Imaging Kit (Blue/Green) 500/530; 350/461 2 × 10 mL $120

Documents

Protocol (PDF): A020
MSDS (PDF):  MSDS-A020
COA (PDF): COA-A020