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EasyProbe™ ActinRed 555 Stain

Introduction

EasyProbes™ ActinRed 555 Stain is a selective, high-affinity F-actin probe conjugated to the red-orange fluorescent dye tetramethylrhodamine (TMR). Our EasyProbes™ ActinRed 555  Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops of stain solution to the cell slide to stain your cells.

Features

  • High-affinity staining of F-actin with superior specificity compared to antibody methods
  • Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
  • Bright fluorescence signal


Suggestions for use

  • In most cases 2 drops of stain solution and an incubation of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
  • EasyProbes™ ActinRed 555 Stain is excited with a maximum at 540 nm, with an emission maximum at 565 nm. It is detected through standard standard TRITC filter.
Specifications:    
Excitation/Emission: 555/575 nm
Shipping Condition: Ambient
Storage Conditions: 4ºC, protect from light
Unit Size: 5 mL

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price
FP032 EasyProbe™ ActinRed 555 Stain 555/575 5 mL $150

Documents

Protocol (PDF): FP032
MSDS (PDF):  FP032
COA (PDF):  FP032

Reference:

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J Colloid Interface Sci (2015) 445:151-160


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Soukup D, Moise S, Céspedes E, Dobson J, Telling ND, 
ACS Nano (2015) 9:231-240