Intracellular Ca2+ plays a central role in cellular processes ranging from neuronal signaling and exocytosis to muscle contraction and bone formation. The fluorescent Ca2+ indicators have been widely used to study the role of Ca2+ in a specific cellular process.
ABP Biosciences has developed a number of calcium indicators to track Ca2+ concentrations with intense fluorescent signals and a range of wavelength options.
Calcium Indicators Selection Guide
Cat. No.
Product Name
Applic ation
C207, C208, C209
Fluo-2, AM
Cell-permeant Ca2+ indicator, Kd: 230 nM, Ex/Em = 488/515 nm
C213, C214, C215
Fluo-3, AM
Cell-permeant Ca2+ indicator, Kd: 390 nM, Ex/Em = 488/525 nm
C219, C220, C221
Fluo-4, AM
Cell-permeant Ca2+ indicator, Kd: 345 nM, Ex/Em = 494/506 nm
C233, C234, C235
Rhod-2, AM
Cell-permeant Ca2+ indicator, Kd: 570 nM, Ex/Em = 550/578 nm
C238
Coelenterazine
Bioluminescent substrate for luciferases, used for calcium detection and the monitoring of reporter genes.
Figure 1. U2OS cells were seeded in a 96-well plate overnight, then incubated with Fluo-4 AM, followed by imaging with a fluorescence microscope using FITC channel.
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