Peroxidase Substrate: Amplex Red

Introduction

10-Acetyl-3,7-dihydroxyphenoxazine (Known as Amplex Red™ reagent, Trade Mark of Molecular Probes) is regarded as the best fluorogenic substrate for peroxidase, because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H₂O₂ in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. Because H₂O₂ is produced in many different enzymatic reactions, the Amplex Red reagent can be used to detect the activity of many different enzymes.

Specifications

Label:	Resorufin
Ex/Em:	571/585 nm
Detection Method:	Fluorescent
Molecular Formula:	C₁₄H₁₁NO₄
Molecular Weight:	257.25
CAS Number:	119171-73-2
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications HRP (Horseradish Peroxidase) Substrate

To order

Buy	Cat.No.	Product name	Ex/Em (nm)	Size	Price
	C291	Amplex Red	571/585	10 mg	$110

Documents

Datasheet (PDF):	C291
MSDS (PDF):	MSDS-C291

References:

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