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Peroxidase Substrate: Amplex Red

Introduction

10-Acetyl-3,7-dihydroxyphenoxazine (Known as Amplex Red™ reagent, Trade Mark of Molecular Probes) is regarded as the best fluorogenic substrate for peroxidase, because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H2O2 in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. Because H2O2 is produced in many different enzymatic reactions, the Amplex Red reagent can be used to detect the activity of many different enzymes.
 

Specifications

Label: Resorufin
Ex/Em: 571/585 nm
Detection Method: Fluorescent
Molecular Formula: C14H11NO4
Molecular Weight:  257.25
CAS Number: 119171-73-2
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
HRP (Horseradish Peroxidase) Substrate
 

To order

Buy Cat.No. Product name Ex/Em (nm) Size Price
C291 Amplex Red 571/585 10 mg $100

Documents

Datasheet (PDF): C291
MSDS (PDF): MSDS-C291

References:

Design and testing of a fluorescence glucose sensor which incorporates a bioinductive material.
Chen HC, Ahmed J
Biomed Sci Instrum (2004) 40:149-154

Inhibition of skeletal muscle S1-myosin ATPase by peroxynitrite.
Tiago T, Simão S, Aureliano M, Martín-Romero FJ, Gutiérrez-Merino C
Biochemistry (2006) 45:3794-3804

The production of reactive oxygen species in intact isolated nerve terminals is independent of the mitochondrial membrane potential.
Sipos I, Tretter L, Adam-Vizi V
Neurochem Res (2003) 28:1575-1581